Links & Downloads: Peer Reviewed Publications


Hitchcock T. 2021. Manufacturing of AAV vectors: translational challenges from development to industrialisation. Emerging Topics in Life Sciences. 5 (5): 725–728.


Ramasamy M N. et al. 2020. Safety and immunogenicity of ChAdOx1 nCoV-19 vaccine administered in a prime-boost regimen in young and old adults (COV002): a single-blind, randomised, controlled, phase 2/3 trial. The Lancet. 396(10267): 1979-1993.


Castiñeiras TS, Williams S, Hitchcock T, Cole JA, Smith DC and Overton TW. 2018. Development of a generic β-lactamase screening system for improved signal peptides for periplasmic targeting of recombinant proteins in Escherichia coli. Scientific Reports.

Castiñeiras TS, Williams S, Hitchcock T, and Overton TW. 2018. Optimizing host cell physiology and stress avoidance for the production of recombinant human tumour necrosis factor α in Escherichia coli. Microbiology.


Aronin A, Amsili S, Prigozhina TB, Tzdaka K, Shen R, Grinmann L, Szafer F, Edebrink P, Rauvola M, Shani N and Dranitzki Elhalel M. 2014. Highly efficient, In-vivo Fas-mediated Apoptosis of B-cell Lymphoma by Hexameric CTLA4-FasL. Journal of Hematology & Oncology. 7:64.

Sibley L, Reljic R, Radford DS, Huang JM, Hong HA, Cranenburgh RM, Cutting SM. 2014. Recombinant Bacillus subtilis spores expressing MP T64 evaluated as a vaccine against tuberculosis in the murine model, FEMS Microbial Lett. 358(2): 170-9.


Cranenburgh RM 2013. Operator-Repressor Titration: Stable Plasmid Maintenance without Selectable Marker Genes. In Minicircle and Miniplasmid DNA Vectors. Wiley-Blackwell. p. 7-21.

Pohl S, Bhavsar G, Hulme J, Bloor AE, Misirli G, Leckenby MW, Radford DS, Smith W, Wipat A, Williamson ED, Harwood CR and Cranenburgh RM, 2013. Proteomic analysis of Bacillus subtilis strains engineered for improved production of heterologous proteins. Proteomics [Epub ahead of print].


Leckenby MW, Spear AM, Neeson BN, Williamson ED, Cranenburgh RM and Atkins HS. 2009. Enhanced vaccine antigen delivery by Salmonella using antibiotic-free operator–repressor titration-based plasmid stabilisation compared to chromosomal integration. Microbial Pathogenesis. 46: 201-206


Harwood CR and Cranenburgh RM, 2008. Bacillus protein secretion: an unfolding story. Trends Microbiol. 16: 73-79.


Bloor AE and Cranenburgh RM, 2006. An efficient method of selectable marker gene excision by Xer recombination for gene replacement in bacterial chromosomes. Appl. Environ. Microbiol. 72: 2520-2525.


Durany O, Bassett P, Weiss AME, Cranenburgh RM, Ferrer P, Lopez-Santin J, de Mas C and Hanak JAJ, 2005. Production of fuculose-1-phosphate aldolase using operator-repressor titration for plasmid maintenance in high cell density Escherichia coli fermentations. Biotechnol. Bioeng. 91: 460-467.


Cranenburgh RM, 2004. An equation for calculating the volumetric ratios required in a ligation reaction. Appl. Microbiol. Biotechnol., 65: 200-202.

Cranenburgh RM, Lewis KS and Hanak JAJ, 2004. The effect of plasmid copy number and lac operator sequence on antibiotic-free plasmid selection by Operator-Repressor Titration in Escherichia coli. J. Mol. Microbiol. Biotechnol. 7: 197-203.


Cooke GD, Cranenburgh RM, Hanak JAJ and Ward JM, 2003. A modified Escherichia coli protein production strain expressing staphylococcal nuclease, capable of auto-hydrolysing host nucleic acid. J. Biotechnol. 101: 229-239.


Cooke GD, Cranenburgh RM, Hanak JAJ, Dunnill P, Thatcher DR and Ward JM, 2001. Purification of essentially RNA free plasmid DNA using a modified Escherichia coli host strain expressing ribonuclease A. J. Biotechnol. 85: 297-304.

Cranenburgh RM, Hanak JAJ, Williams SG, and Sherratt DJ, 2001. Escherichia coli strains that allow antibiotic-free plasmid selection and maintenance by repressor titration. Nucleic Acids Res. 29: e26.

Hanak JAJ and Cranenburgh RM, 2001. Antibiotic free plasmid selection and maintenance in bacteria. In Recombinant protein production with prokaryotic and eukaryotic cells. Kluwer Academic Publishers. p. 111-124.


Varley DL, Hitchcock AG, Weiss AME, Horler WA, Cowell R, Peddie L, Sharpe GS, Thatcher DR, Hanak JAJ, 1999. Production of plasmid DNA for human gene therapy using modified alkaline cell lysis and expanded bed anion exchange chromatography. Bioseparation 8: 209-217.


Williams SG, Cranenburgh RM, Weiss AME, Wrighton CJ, Sherratt DJ and Hanak JAJ, 1998. Repressor titration: a novel system for selection and maintenance of recombinant plasmids. Nucleic Acids Res. 26: 2120-2124.